https://www.british-history.ac.uk/vch/cambs/vol10/pp470-472
Landwade: - Manors
Landwade continued: For the next four centuries the manor remained in the possession of the Cotton family who built up a substantial estate including land in Exning and Fordham. By 1436 Thomas Cotton had died, and in 1437 Walter (d. 1445) settled it upon his son and heir William. (fn. 24) William held the manor, and on his death in 1455, it passed to his son Thomas (d. 1499). (fn. 25) His son and heir Sir Robert (d. 1517) succeeded. (fn. 26) He was followed by his son, John (later knighted), a minor in 1519, whose half-brother Thomas Griffith had custody of the manor. (fn. 27) In the 1520s, after Sir Robert's son John took possession of Landwade and Exning, his paternal uncle and namesake John claimed the manors (fn. 28) Sir John Cotton remained in possession of the manor from the 1520s until his death in 1593 at the age of 83, when he was succeeded by his son Sir John (d. 1620). (fn. 29) On his death his heir, also called John, was a minor, aged five. His mother Anne and her second husband Sir John Carleton held the manor until Carleton's death in 1637, when John Cotton took possession.
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England & Wales, Prerogative Court of Canterbury Wills, 1384-1858
Name: John Cotton [Johis Cotton ]
Probate Date: 21 Jun 1594
Residence: Landwade , Cambridgeshire, England
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HERE LIETH INTOMBED THE BODIE OF SIR JOHN COTTON KNIGHTE SONNE OF SIR ROBERTE COTTON KNIGHTE AND ALSO THE BODIE OF DAME ISABELL WIFE OF THE SAIDE SIR JOHN AND DAVGHTER OF SIR WILLIAM SPENCER KNIGHTE
WHICHE SIR JOHN DIED THE 21 DAIE OF APRILL 1593 INTHE YEARE OF HIS AGE 81. AND THE SAIDE DAME ISABELL DIED THE 2 DAIE OF NOVEMBER 1578 INTHE YEARE OF HER AGE 63
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The History of Parliament online. From the following source:
The History of Parliament: the House of Commons 1509-1558, ed. S.T. Bindoff, 1982
Sir John Cotton made his will 8 Feb 1593, died on the following 21 Apr. and was buried in Landwade church, where monuments commemorate him and his wife. After charitable gifts and legacies to friends, relatives and servants, he left £100 to each of his two surviving younger sons, £200 to his grandchildren by his son Robert, and the residue to his eldest son John, the sole executor. A verbal codicil made five weeks before Cotton’s death purported to transfer the £200 left to his grandchildren to their father, but was later held invalid; probate of the will was granted on 21 June 1594.(6)
(6)Transactions of the Cambridgeshire and Huntingdonshire Archaeological Society
Volume: 4.5 (1921), p 122

A Who’s Who of Tudor Women
ISABEL SPENCER (1515-November 2, 1578)
Isabel Spencer was the daughter of Sir William Spencer of Althorp, Northamptonshire (d. June 22, 1532) and Susan Knightley (d.1539). In his will, proved July 8, 1532, her father provided for his daughters until they married and left each of them five hundred marks, so long as they heeded the advice of his executors in choosing their husbands. Isabel’s mother was one of the executors. Isabel married Sir John Cotton of Landwade and Chevveley, Cambridgeshire (1512/13-April 21, 1593). They had eight sons and five daughters, including John (c.1543-1620/1), Frances, Robert, Alice, Anne, Sarah, and Edmund. Portrait: effigy in the north chapel at Landwade.